Regulation of Transcriptional Activation and Chromatin Architecture

Understanding how the interplay among the macromolecular complexes of RNA Polymerase II transcription machinery regulates transcription will critically benefit from the detailed structural information about the mechanism of their functional interactions. However, because of its large size and complexity and it is stabilized by a multiplicity of dynamic protein-protein and protein-nucleic acid interactions, the structural analysis is complicated by both compositional and conformational heterogeneity.

Cryo electron microscopy (cryo-EM) is a powerful technique to study both the structure and dynamics of macromolecular complexes in their close to native state. We are interested in examining the structures and functional interactions of key macromolecular complexes responsible for regulation of eukaryotic transcription and chromatin structure, aiming at a structural understanding of the molecular mechanism.